Dochinpira 2 (1993)

The Haozakura-kai continues to relentlessly tail Jin every day, but now, a yakuza from the Enshin-gumi, Mizuno Tetsuo, appears. Just as they share a cup of sake as blood brothers, an assassin from the Tendo-gumi attacks and kills Mizuno, leaving behind his only sister, Rei. This is where Jin's days of agony begins, entrusted with Rei.